[Parameters: Topic='BIOSPHERE', Term='AQUATIC ECOSYSTEMS']
Viral abundance and productivity in the Southern OceanEntry ID: BROKE-West_viralabundance
Abstract: Data acquisition
Samples were collected at the 3 sites at 9 different depths.
Depths included the bottom depth as well as 8 samples in the top 200M.
50ml samples were collected from the niskin bottles attached to the CTD.
20ml was filtered through a 0.02 microlitre filter to remove the viruses and larger organisms from the sample.
1ml of this virus free water was then pipetted into individual ... cryovials.
A further 1ml was then taken from the original seawater sample and was then passed through a 0.2 microlitre filter, which retained all the bacteria and algae.
The 0.2 microlitre filter was then placed in the cryovial with the 1ml of virus free seawater.
Samples were then fixed with glutaraldehyde (5 microlitre, 50% concentration) at hourly intervals for 4 hours.
After fixation samples were placed in liquid nitrogen and later stored at -80 degrees C.
All samples will be analysed using a flowcytometer.
The fields in these datasets are:
This work was completed as part of ASAC projects 2655 and 2679 (ASAC_2655, ASAC_2679).
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This data set description is a member of a collection. The collection is described in
Start Date: 2006-01-10Stop Date: 2006-02-28
Quality Samples were taken at 3 stations along leg 1.
9 depths were sampled at the 3 sites and depths were chosen based on the fluorescence maximum on the downcast of the CTD.
Access Constraints These data are publicly available from the provided URL.
Use Constraints This data set conforms to the PICCCBY Attribution License
Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-We... when using these data.
Data Set Progress
Distribution Media: HTTP
Distribution Size: 44 kb
Distribution Format: Excel, csv
Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Phone: +61 8 8201 3684
Fax: +61 8 8201 3015
Email: jim.mitchell at flinders.edu.au
School Of Biological Sciences GPO BOX 2100 FLINDERS UNIVERSITY OF SA
Province or State: South Australia
Postal Code: 5001
Role: TECHNICAL CONTACT
Phone: +33 3 2199 2937
Email: Laurent.Seuront at univ-lille1.fr
28 AVENUE FOCH STATION MARINE DE WIMEREUX
Postal Code: 62930
Creation and Review Dates
DIF Creation Date: 2006-03-10
Last DIF Revision Date: 2012-05-04